[onelife]

Hey all.. I have been reading some great things about CLA and I was wondering if anyone has been using it and what there thoughts on it were?
I am planning on competing in the somewhat near future and I am trying to stay as lean as possible while putting on another 3 or 4 pounds. Thought this might be a good one to add to the arsenal. Any opinions? thanks!!

[onelife]

bump... no one uses it?!?!

[nightop]

no.

[ScottL]

use search engine for negative info posted previously.

[Deserusan]

The research isn't very compelling in your under 15% bodyfat groups.

[onelife]

well saves me some money than... thank you Deserusan

[methodice]

CLA still seems to be bought alot, even nowadays. I enquired over at BB.com if anyone had new data on it, as I stopped looking into it when they showed positive effects of certain isomers and negative affects of the other isomers. Basically until someone manufactures isolated isomers to the public, CLA is not recommended as far as I know.

[onelife]

thanks a lot MD. I have also seen some positive studies done on it..but not from the most credible sources.

[Rodzilla]

I have not used CLA, and since I have realized it is a transfat I do not use it, despite reported benefits.

[Maltese727]

I've tried Optimum Nutrition CLA didn't really notice much from 1 bottle, i think your better off with something else.

[Primordial Performance]

Im a big fan of CLA, in fact we will probably be picking it up as a product soon.

It has more positive human research than any fat burning supplement aside from ephedra type products. If you take enough (about 5-6gm of pure CLA) the results can be quite impressive. It’s a naturally occurring trans-fat and its one of the few trans fats that are actually healthy.


-Pp

[SteveSliwa]

Rodzilla, on Oct 21 2007, 11:09 AM, said:

I have not used CLA, and since I have realized it is a transfat I do not use it, despite reported benefits.

Actually it's not a quite the same as a transfat and thus does not require listing by the FDA. You consume it everything you drink milk and meat.

[Colin]

Primordial Performance, on Dec 30 2007, 02:21 PM, said:

Im a big fan of CLA, in fact we will probably be picking it up as a product soon.

It has more positive human research than any fat burning supplement aside from ephedra type products. If you take enough (about 5-6gm of pure CLA) the results can be quite impressive. It’s a naturally occurring trans-fat and its one of the few trans fats that are actually healthy.
-Pp

Please clarify what you mean WRT pure CLA.

The bulk of scientific evidence shows the CLA that is now commercially available to be either as:

1)Largely self defeating via isomer content.

2)Potentially lead to fat gain due to the manipulation of glucose leading to a diabetic like state e.g.highly undesirable and actually the direct opposite end result that CLA is marketed towards,fat loss.

3)Inducing a negligible amount of fat loss

I do agree if the rigbht isomers were present in a CLA supplement that it would be very impressive,especially in conjunction with sesamin but such CLA is not offered for sale.

[SteveSliwa]

Colin, on Dec 30 2007, 05:32 PM, said:

Please clarify what you mean WRT pure CLA.

The bulk of scientific evidence shows the CLA that is now commercially available to be either as:

1)Largely self defeating via isomer content.

2)Potentially lead to fat gain due to the manipulation of glucose leading to a diabetic like state e.g.highly undesirable and actually the direct opposite end result that CLA is marketed towards,fat loss.

3)Inducing a negligible amount of fat loss

I do agree if the rigbht isomers were present in a CLA supplement that it would be very impressive,especially in conjunction with sesamin but such CLA is not offered for sale.

LEF claims their product is (it does have sesamin).

[Primordial Performance]

Colin, on Dec 30 2007, 03:32 PM, said:

Please clarify what you mean WRT pure CLA.

The bulk of scientific evidence shows the CLA that is now commercially available to be either as:

1)Largely self defeating via isomer content.

2)Potentially lead to fat gain due to the manipulation of glucose leading to a diabetic like state e.g.highly undesirable and actually the direct opposite end result that CLA is marketed towards,fat loss.

3)Inducing a negligible amount of fat loss

I do agree if the rigbht isomers were present in a CLA supplement that it would be very impressive,especially in conjunction with sesamin but such CLA is not offered for sale.

A 50/50 mix between cis9, trans11 and trans10, cis 12 seems most desirable based on what I’ve seen. (readily available on the market)

A recent meta-analysis by the American Journal of Clinical Nutrition found a very clear positive correlation to fat loss after reviewing 18 CLA studies. (an average of .2 lbs of fat lost per week at 3.2gm day – w/ a 50/50 isomer mix) I don’t think there is any doubt in the scientific community that a sufficient dose of CLA helps with fat loss.

In regards to insulin sensitivity -

Insulin resistance—associated cardiovascular disease: potential benefits of conjugated linoleic acid
Denise V Aminot-Gilchrist and Hope DI Anderson
Am. J. Clinical Nutrition, Jun 2004; 79: 1159S - 1163S.

-Pp

[SteveSliwa]

Primordial Performance, on Dec 30 2007, 06:29 PM, said:

A 50/50 mix between cis9, trans11 and trans10, cis 12 seems most desirable based on what I’ve seen. (readily available on the market)

A recent meta-analysis by the American Journal of Clinical Nutrition found a very clear positive correlation to fat loss after reviewing 18 CLA studies. (an average of .2 lbs of fat lost per week at 3.2gm day – w/ a 50/50 isomer mix) I don’t think there is any doubt in the scientific community that a sufficient dose of CLA helps with fat loss.

In regards to insulin sensitivity -

Insulin resistance—associated cardiovascular disease: potential benefits of conjugated linoleic acid
Denise V Aminot-Gilchrist and Hope DI Anderson
Am. J. Clinical Nutrition, Jun 2004; 79: 1159S - 1163S.

-Pp

I agree with you. But I know alot of people won't.

[Jakeshorts]

no one else is saying it... so I will - .2lbs a week are impressive results?

[SteveSliwa]

Jakeshorts, on Dec 30 2007, 07:06 PM, said:

no one else is saying it... so I will - .2lbs a week are impressive results?

It's something and consider what happens when you add other compounds to enhance it's effects.

[Colin]

I haven't looked into LEF's CLA/sesamin supp yet but I will check on their claims.The fact that sesamin is included in it isn't a big deal in itself (considering how cheap bulk sesamin is) but if it is the OPTIMAL mix of isomers then hell yeah,that would be a big deal,so to speak.

LEF's price tag on that particular supplement would definitely be worth the money but there are too many studies showing that 50:50 CLA (what is now by most odds the sole commercially available form) is bollocks to merit the expense of what may be at best marginal positive results.So I can't extend that to any CLA other than the LEF item with the stipulation that if the claims they have made are in fact true.

Jake,a bit shy of a quarter of a pound of fat loss in a week isn't remotely impressive.

Offhand several supplements yield much more than that,old school/inexpensive as hell E/C at 12.5mg E 100mg C thrice daily or HEAT will yield 2-4 times that figure of .2 pound of fat loss per week.

I may or may not dig up some studies on CLA's more than fair chance of negative side effects,considering how old and beaten down this topic is I'd rather not spend the time and effort to do so.

A search under CLA with the user names of Loki or Par Deus,with preference given to Loki as I recall he was adamant regarding the severity of just how hardly CLA blows with numerous referenced studies.

Searching avantlabs.com/forum would probably show these threads or this forum may have them,I am not sure as they were all produced well before the Avant/MM boards split.

If no one else volunteers I'll do the leg work,but not tonight.

[ScottL]

This was discussed in the last few weeks including a link to a previous post where i've posted a whole bunch of concerning abstracts as well as a link to the Par/Loki dialog of interest.

[Jakeshorts]

Colin, on Dec 30 2007, 08:28 PM, said:

I haven't looked into LEF's CLA/sesamin supp yet but I will check on their claims.The fact that sesamin is included in it isn't a big deal in itself (considering how cheap bulk sesamin is) but if it is the OPTIMAL mix of isomers then hell yeah,that would be a big deal,so to speak.

LEF's price tag on that particular supplement would definitely be worth the money but there are too many studies showing that 50:50 CLA (what is now by most odds the sole commercially available form) is bollocks to merit the expense of what may be at best marginal positive results.So I can't extend that to any CLA other than the LEF item with the stipulation that if the claims they have made are in fact true.

Jake,a bit shy of a quarter of a pound of fat loss in a week isn't remotely impressive.

Offhand several supplements yield much more than that,old school/inexpensive as hell E/C at 12.5mg E 100mg C thrice daily or HEAT will yield 2-4 times that figure of .2 pound of fat loss per week.

I may or may not dig up some studies on CLA's more than fair chance of negative side effects,considering how old and beaten down this topic is I'd rather not spend the time and effort to do so.

A search under CLA with the user names of Loki or Par Deus,with preference given to Loki as I recall he was adamant regarding the severity of just how hardly CLA blows with numerous referenced studies.

Searching avantlabs.com/forum would probably show these threads or this forum may have them,I am not sure as they were all produced well before the Avant/MM boards split.

If no one else volunteers I'll do the leg work,but not tonight.

the voice of reason. Thank you Colin....

OTOH - brand new from the pub - found this researching mytostatin inhibition -


Conjugated linoleic acid suppresses myogenic gene expression in a model of human muscle cell inflammation.

Larsen AE, Cameron-Smith D, Crowe TC.
School of Exercise and Nutrition Sciences, Deakin University, Burwood, Victoria 3125, Australia.
Proinflammatory cytokines, such as tumor necrosis factor (TNF)-alpha, contribute to muscle wasting in inflammatory disorders, where TNFalpha acts to regulate myogenic genes. Conjugated linoleic acid (CLA) has shown promise as an antiproliferative and antiinflammatory agent, leading to its potential as a therapeutic agent in muscle-wasting disorders. To evaluate the effect of CLA on myogenesis during inflammation, human primary muscle cells were grown in culture and exposed to varying concentrations of TNFalpha and the cis-9, trans-11 and trans-10, cis-12 CLA isomers. Expression of myogenic genes (Myf5, MyoD, myogenin, and myostatin) and the functional genes creatine kinase (CK) and myosin heavy chain (MHC IIx) were measured by real-time PCR. TNFalpha significantly downregulated MyoD and myogenin expression, whereas it increased Myf5 expression. These changes corresponded with a decrease in both CK and MHC IIx expression. Both isomers of CLA mimicked the inhibitory effect of TNFalpha treatment on MyoD and myogenin expression, whereas myostatin expression was diminished in the presence of both isomers of CLA either alone or in combination with TNFalpha. Both isomers of CLA decreased CK and MHC IIx expression. These findings demonstrate that TNFalpha can have specific regulatory effects on myogenic genes in primary human muscle cells. A postulated antiinflammatory role of CLA in myogenesis appears more complex, with an indication that CLA may have a negative effect on this process.

[Whacked]

http://www.mindandmu...showtopic=31603

[Primordial Performance]

Jakeshorts, on Dec 30 2007, 05:06 PM, said:

no one else is saying it... so I will - .2lbs a week are impressive results?

Yes, it is impressive jake, and it’s on the lower end of the spectrum compared to the results obtained from higher doses of CLA.

You will find some negative studies from ultra-high doses of CLA in mice and a couple human studies saying it has no effect on body fat (at a lower dose), but the majority of human research shows that a relevant dose is positively correlated to fat loss and overall health.

Hell, Jamba Juice just picked it up for their new drink line... now if that isnt proof I dont know what is. ;-}

-Pp

[Jakeshorts]

supplement companies marketing hype don't prove the supplement sells. It proves the hype sells...

we'll have to agree to disagree with .2lbs PER WEEK being impressive or even noticable IMHO. I bet your own t3 product over shadows that several times over.

for all our benefit:

http://www.mindandmuscle.net/forum/index.p...mp;#entry138705

http://www.avantlabs.com/forum/index.php?s...l=cla+oxidation

[SteveSliwa]

Jakeshorts, on Dec 30 2007, 09:02 PM, said:

supplement companies marketing hype don't prove the supplement sells. It proves the hype sells...

we'll have to agree to disagree with .2lbs PER WEEK being impressive or even noticable IMHO. I bet your own t3 product over shadows that several times over.

for all our benefit:

http://www.mindandmuscle.net/forum/index.p...mp;#entry138705

http://www.avantlabs.com/forum/index.php?s...l=cla+oxidation

I don't think it's impressive rather an add on based on the research.

[Colin]

Directly below is a single study taken from one of the two threads linked(thanks J) and there's plenty more within those two threads to make anyone reevaluate their take on CLA.

After the study is a post by Trouble,detailing her overwhelmingly negative (and lengthy) run with it.For those who don't know of her postings,she was on the fucking ball WRT advanced scientific theory.


Arterioscler Thromb Vasc Biol. 2006 Feb;26(2):307-12. Epub 2005 Dec 8. Related Articles, Links

Conjugated linoleic acid impairs endothelial function.

Taylor JS, Williams SR, Rhys R, James P, Frenneaux MP.

Morriston Hospital, Swansea, UK. jswt@totalise.co.uk

OBJECTIVE: To determine the effect of dietary supplementation with conjugated linoleic acid (CLA) on body mass index (BMI), body fat distribution, endothelial function, and markers of cardiovascular risk. METHODS AND RESULTS: Forty healthy volunteers with BMI >27 kg/m2 were randomized to receive a CLA isomeric mixture or olive oil in a 12-week double-blind study. Subcutaneous body fat and abdominal/hepatic fat content were assessed using skin-fold thicknesses and computed tomography scanning, respectively. Endothelial function was assessed by brachial artery flow-mediated dilatation (FMD). Plasma isoprostanes were measured as an index of oxidative stress. CLA supplementation did not result in a significant change in BMI index or total body fat. There was a significant decrease in limb (-7.8 mm, P<0.001), but not torso skin-fold thicknesses or abdominal or liver fat content. Brachial artery FMD declined (-1.3%, P=0.013), and plasma F2-isoprostanes increased (+91 pg/mL, P=0.042). CONCLUSIONS: A CLA isomeric mixture had at most modest effects on adiposity and worsened endothelial function. On the basis of these results, the use of the isomeric mixture of CLA as an aid to weight loss cannot be recommended




Troubles post.....................






What about anecdotal evidence?

I used Tonalin for a period of two years. At first, it seemed to work, but nothing along the lines of Sesathin in terms of efficacy. After a period of about 1 year (yes, I cycled use every two months to avoid accomodation effects), I began to notice that even with dedicated aerobic exercise, my weight control was slipping, despite maintaining the same diet and exercise habits. I attributed it to stress, but on hind site, it may well have been deteriorating insulin sensitivity. Once I saw the abstracts on CLA, I ditched its use. I have some 100 caps 1 gram caps that I finally pitched, mostly in anger once I realized the hype and con behind it. Long term use, and the 50:50 mix of isomers, even when using sensibile lifestyle habits, were apparently enough to knock my insulin sensitivity into the shitter.

Moreover, the reports of worsened inflammation are correct. I developed exercise-related asthma during this time period, despite rigorous stress control and careful attention to diet, exercise and sleep patterns. During the last few weeks of asthma attacks, they also occured at night usually around 2-3am), which is truely terrifying.

I stopped use of this supp, and within a period of three weeks, my asthma attacks, which had appeared several months earlier and slowly grown worse in intensity and frequency, diminished and eventually ceased.

I do not recommend that anyone consider use CLA.

[SteveSliwa]

I think the differences in effects/studies are due mostly the different formulations of CLA on the market.

Here's a good link to various studies: http://www.wisc.edu/fri/clarefs.htm

[Primordial Performance]

Anyone know how much (dosage) troubles was using?

Perhaps the practical take home message here is to supplement your high-dose, long-term CLA with omega-3's and insulin sensitizers...

7-keto anyone? Fatty liver? Lets be fair here… A balancing effort should accompany virtually any supplement that has such a marked physiological impact.

-Pp

[Colin]

Primordial Performance, on Dec 30 2007, 10:48 PM, said:

Anyone know how much (dosage) troubles was using?

Perhaps the practical take home message here is to supplement your high-dose, long-term CLA with omega-3's and insulin sensitizers...

7-keto anyone? Fatty liver? Lets be fair here… A balancing effort should accompany virtually any supplement that has such a marked physiological impact.

-Pp

I'm not sure about the dosage Trouble used.

I think it is a really poor idea to suggest that CLA should be used in high dosages to begin with,let alone long cycles.Allowing for what I view as a far fetched idea of negating the sides via 7-keto and insulin sensitizers as who's to say with any real clarity as to wether this would really be completely effective in crushing all sides,why even tamonkley around in an insipid attempt to avoid side effects from what is at the very best a marginally effective fat loss supplement?

.And yes,complete eradication of all sides would be called for,considering the financial cost to benefit ratio,given that we are going on a figure of less than a 1/4 pound per week of fat loss.CLA is not remotely comparable to a supp/drug that induces such a signifigant amount of fat oxidation as to require "on cycle protection",IOW this is clearly not DNP-esque or a steroidial compound for that matter.

If one wants non-stimulatory fat loss,GTE and sesamin are the safe and proven route that actually convey multiple health benefits.A brief run down being anti-oxidant via GTE,sesa is anti-imflammatory while CLA is imflammatory.Sesa also has a slew of other well established benefits on health,improved choelesterol profile as signifigantly to be along the lines of Lipitor and kidney/liver protectant action among others.Either of them alone would trump the .2 pound fat loss by far, so where is the justification in using highly dosed ( read:expensive) to have other fat loss supps added to potentiate such a diminished effect?

Clearly,an insulin sensitizer (refering to apple cider vineger as it is very effective in this regard as well as quite affordable) and fish oil are good choices to use regardless of CLA.For the sake of irony,I will also mention that sesamin also acts in a fairly strong,positive manner towards a favorable insulin profile.

In fairness to the consumer,new compounds must be researched and isolated if supplement companies want to create new avenues of revenue,rather than re-tool what does obviously give little benefit for a very high cost,in more ways than one.

Here's what I'm alluding to,to start with:




J Lipid Res. 2003 Dec;44(12):2234-41. Epub 2003 Aug 16. Related Articles, Links


Efficacy and safety of dietary supplements containing CLA for the treatment of obesity: evidence from animal and human studies.

Larsen TM, Toubro S, Astrup A.

Department of Human Nutrition, Center for Advanced Food Studies, The Royal Veterinary and Agricultural University, DK-1958 Frederiksberg C, Denmark. tml@kvl.dk

Dietary supplements containing conjugated linoleic acid (CLA) are widely promoted as weight loss agents available over the counter and via the Internet. In this review, we evaluate the efficacy and safety of CLA supplementation based on peer-reviewed published results from randomized, placebo-controlled, human intervention trials lasting more than 4 weeks. We also review findings from experimental studies in animals and studies performed in vitro. CLA appears to produce loss of fat mass and increase of lean tissue mass in rodents, but the results from 13 randomized, controlled, short-term (<6 months) trials in humans find little evidence to support that CLA reduces body weight or promotes repartitioning of body fat and fat-free mass in man. However, there is increasing evidence from mice and human studies that the CLA isomer trans-10, cis-12 may produce liver hypertrophy and insulin resistance via a redistribution of fat deposition that resembles lipodystrophy. CLA also decreases the fat content of both human and bovine milk. In conclusion, although CLA appears to attenuate increases in body weight and body fat in several animal models, CLA isomers sold as dietary supplements are not effective as weight loss agents in humans and may actually have adverse effects on human health.

PMID: 12923219 [PubMed - in process]


Refer to Sphlonged comments in this thread on high dosing CLA in the 15-30 gram range showing fat loss but with signifigant health consequences:

http://www.mindandmuscle.net/forum/index.p...mp;#entry138705

IOW,I am not alone in my position and any careful consideration yields allies on this issue.

I do believe that Nightop's one word reply of "no." within this thread on the question of CLA supplementation was sufficient but then again,I do enjoy a good debate as much as the next sap

The sole outstanding reason I am interested in CLA is in the context of the proper isomer in conjunction with sesamin as Steve posted this to be the case with LEF's new CLA supp.Then again,fucoxathin,sesamin,GTE and a couple other cough,cough VERY SAFE supps would convey such fat loss benefit as the ideal isomer content CLA coupled with sesamin.

If you want to take chances and deal with some sort of adverse health effect to one extent or another,at least use something that will burn a lot of fat a'la DNP.This can be easily sourced off a Google search from chemical suppliers at 100 grams $50-60.Or buy it on the secondary market letting someone else do the work as far as capping is concerned and pay them $2 per 200mg cap.


Whatever is decided upon,CLA should not be included.

[SteveSliwa]

Several people don't have a remarkable positive viewpoint on CLA in weight loss when used alone. I personally don't recommend CLA alone to lose weight.

Weight Loss and Other Benefits of Conjugated Linoleic Acid
By Dale Kiefer

Conjugated linoleic acid (CLA) is gaining the attention of a public grown ravenous for new weapons to wield in the never-ending “battle of the bulge.” You may have heard some of the hype, but what is the real story behind this relative newcomer to the weight-loss market? A growing body of research reveals that CLA is a potent nutrient responsible for a variety of important health benefits, from fighting cancer and diabetes to boosting fat loss and building lean muscle.

First hailed for its striking anti-cancer effects,1 CLA is now the subject of intensive research by scores of scientists around the globe, all of them intrigued by this nutrient’s multiple human health benefits. Accelerated fat loss is just one of several boons that have been ascribed to this fatty acid supplement. CLA’s other reported benefits include combating diabetes, building lean muscle mass, and thwarting cancer at each major stage of its development.1-7

While it may be too soon to render the final verdict on all of CLA’s purported benefits, the scientific evidence clearly supports some of its biological effects. In this article, we will examine the current state of CLA research, covering this important nutrient’s demonstrated effects and examining others that remain to be fully elucidated.

What Is CLA?

CLA comprises several chemically similar compounds, or isomers, derived from the fatty acid known as linoleic acid. Present in dairy products and the meat of ruminant animals—livestock such as cattle and sheep that chew their cud—CLA is believed to play an important role in modulating immunity and regulating lipid metabolism.2,5,8

The “conjugated” portion of this nutrient’s name refers to two double chemical bonds within its structure. The location of the bonds dictates a given isomer’s specific designation. For example, the cis-9, trans-11 CLA isomer (also known as rumenic acid, or c9t11) is the most common isomer found in ruminant-derived foods, and is believed to be one of the most biologically active isomers. Trans-10, cis-12 CLA (t10c12) is present in far smaller concentrations, but may play an especially important role in modulating health.4 These two isomers are considered the most biologically important forms of CLA for human health. Most commercially available CLA supplements include a roughly equal mixture of both.

CLA and Weight Loss

Early CLA experiments on laboratory rodents generated considerable surprise and excitement. Scientists discovered that CLA, when added to the diet, promotes dramatic and relatively rapid weight loss in a variety of lab animals. Perhaps more important, the animals lost body fat while increasing their lean muscle mass.

When researchers fed rabbits a diet that normally promotes atherosclerosis, the animals were considerably less likely to develop this artery-clogging disease when CLA was included in their diet. Their total cholesterol and LDL (low-density lipoprotein) levels dropped, as did their triglyceride values.9 Furthermore, using a strain of rats bred for a tendency to develop obesity-related type II diabetes, scientists were able to significantly delay the onset of diabetes by adding CLA to the animals’ diet.10

Needless to say, scientists were excited about CLA’s potential to fight fat in humans. Obesity is epidemic in much of the developed world, and the federal Centers for Disease Control and Prevention reports that approximately two thirds of American adults are now overweight or obese. Even more alarming is that 13% of American children and adolescents are overweight.11,12 All that excess tonnage takes a heavy toll, not only on individuals’ health but also on our collective bottom line. In 2002, the estimated total economic cost of obesity was about $93 billion.13

It is understandable that hopes for CLA ran high in those early days of model-thin mice and shrinking rats. However, CLA may not be quite the magic bullet scientists had hoped it would be. Although CLA triggers dramatic fat loss in rodents, the effect is less pronounced in humans. Some human clinical trials have reported promising results,14 while others have found little or no fat-loss effect.17 A few studies have reported adverse effects of CLA on lipid profiles, markers of oxidative stress, and glucose handling,14 while other long-term studies have not observed these negative effects.21

Thus, our understanding of CLA is in a state of flux. Much work remains to be done as we assess CLA’s potential benefits or risks. With that caveat, let us shed some light on what is known about this important nutrient.

CLA and Diabetes

CLA appears to prevent the development of type II diabetes (often associated with obesity) in rats bred for their propensity to grow abnormally overweight. Some researchers have proposed that the t10c12 isomer is primarily responsible for this anti-diabetic and anti-obesity effect.14

Human studies confirm the efficacy of the t10c12 isomer in promoting weight loss. For example, in 2003, Swedish researchers published the results of a series of controlled experiments in which subjects received either a CLA mixture or purified t10c12 isomer. The results indicated that CLA does reduce body fat, though no overall weight loss was detected. Unexpectedly, the pure t10c12 isomer appeared to adversely affect glucose and lipid levels, and to impair insulin sensitivity.14

Other research, however, suggests that CLA may improve the insulin sensitivity of fat cells, and may work in combination with nutrients such as biotin and coenzyme Q10 to prevent diabetes.15

A recent study showed that eight weeks of CLA supplementation increased insulin sensitivity in young, sedentary humans. Ten participants took 4 grams of CLA daily. Six of the subjects experienced large increases in insulin sensitivity, while two showed no change and two demonstrated a decrease in insulin sensitivity. These results suggest that response to CLA varies among individuals, with many responding favorably.16

In 2000, researchers in California conducted an experiment on 17 women who were confined to a metabolic laboratory for three months. Their activity levels and food intakes were strictly controlled. After a month of baseline monitoring, the subjects were given a daily supplement of 3 grams of either a CLA mixture or an inactive placebo. After two months of supplementation, the scientists concluded that CLA had no effect on body composition.17 The study’s design did not, however, account for the possibility that CLA may reduce body fat or weight by suppressing appetite, since all of the subjects in this trial were required to adhere to a uniform diet. It is also possible that the subjects simply did not consume enough CLA or did not consume it long enough to generate significant changes in body composition.

Reducing Body Fat
Other researchers have reported that CLA does indeed reduce body fat, if not overall weight. In a double-blind experiment, a leading researcher gave 21 patients with type II diabetes a daily dose of 8 grams of either a CLA mixture or placebo. After two months, the researchers correlated the subjects’ CLA blood levels with changes in their weight. They discovered that the t10c12 isomer—but not the c9t11 isomer—was significantly associated with weight loss.10 Intriguingly, the t10c12 isomer, which scientists believe may exert the most potent effects on body composition, is also associated with a decrease in leptin, a hormone associated with the body’s hunger-satiety feedback mechanism. Interestingly, the amount of CLA used in this study, 8 grams per day, is more than twice the amount given to subjects in trials in which no significant fat loss was reported.10

Other research, however, indicates that 2-4 grams of CLA taken daily may be enough to trigger fat loss. Several Scandinavian studies over the last five years offer evidence that relatively low doses of CLA reduce body fat, if not overall weight. A Norwegian study documented significant body-fat loss with just 1.8 grams of CLA per day. This double-blind, placebo-controlled, randomized study of 20 healthy, normal-weight people lasted three months. Subjects performed standardized exercises for 90 minutes, three times a week. They consumed either a placebo or 1.8 grams of a commercially available CLA mixture, which featured the c9t11 and t10c12 isomers in equal amounts. Subjects taking CLA experienced significant reductions in body fat, as measured by near-infrared light, and the CLA was well tolerated. The placebo subjects, by contrast, showed no change in body fat.18

Another Norwegian controlled study enrolled 60 overweight or obese subjects who were randomly assigned to one of five groups and received daily doses of 1.7-6.8 grams of CLA or placebo. Body fat was measured by dual-energy X-ray absorptiometry at baseline and then at six and 12 weeks. Scientists found a significantly greater body-fat loss among the groups taking 3.4 grams or more of CLA compared to the control subjects. Intriguingly, the group receiving 1.7 grams of CLA experienced no significant body-fat loss, nor did the groups taking more than 3.4 grams of CLA see any additional body-fat loss.19 Researchers concluded that a dose of 3.4 grams results in significant body-fat loss compared to placebo, but that greater amounts of CLA do not result in greater fat loss. The CLA was well tolerated and adverse events were no different among the placebo and various CLA groups. Forty-seven subjects completed the three-month study. Blood lipids and other blood-profile health parameters were unchanged among all groups.19

Slimming News from Sweden

A study in Sweden enrolled 53 healthy men and women who were randomly assigned to receive either 4.2 grams of CLA a day or an equal amount of placebo. Neither the subjects nor the researchers in this double-blind trial knew who was taking which substance. After three months, CLA subjects experienced a significant decrease in their proportion of body fat compared to control subjects. Weight, body mass index, blood glucose, and lipids remained unchanged for both groups. The researchers concluded that CLA reduces body fat without affecting other health parameters.20

A recent report from the University of Wisconsin confirmed CLA’s safety. Obese people who were otherwise healthy received either 6 grams daily of CLA or a placebo for one year. General blood chemistries revealed no adverse effects on liver function, blood glucose, lipids, or insulin levels from long-term CLA supplementation.21

Although the research results remain somewhat mixed, CLA supplementation appears to be safe and potentially beneficial for people seeking to trim body fat.

Battling Cancer at Every Turn
Ironically, the potent cancer-fighter CLA was first identified in fried and grilled beef, foods that are better known for containing cancer-causing substances (such as heterocyclic amines) than for supplying beneficial anti-carcinogens.1,22,23 Yet that is precisely where scientists at the University of Wisconsin first discovered the conjugated isomers they dubbed CLA.1

The Wisconsin scientists discovered that one or more of the isomers inhibit mutations in bacteria. Researchers believe that these mutations, which are generally damaging alterations to the genetic code, underlie the development of many cancers. They wondered whether CLA could offer protection against an experimentally induced laboratory model of skin cancer, and soon turned their attention to a rodent model of human cancer.1

The researchers enlisted the aid of rodents specially bred for their susceptibility to developing skin cancer when provoked with an appropriate toxin. One group of the cancer-prone mice was pre-treated with a synthetic mixture of four CLA isomers. Control groups of mice were pre-treated with either linoleic acid or an organic solvent. The researchers then applied a known carcinogenic chemical to the skin of all the test animals. It was soon apparent that CLA provides considerable protection against experimentally induced tumors, as the control subjects developed twice as many tumors as the CLA-treated mice.1

SESAME LIGNANS ENHANCE CLA’S ACTION

Lignans, the fibrous compounds found in many plant-based foods, possess numerous health-promoting effects. Sesame seed lignans in particular are beneficial. Studies show that sesame lignans, including sesamin and sesaminol, enhance vitamin E’s absorption and availability, improve lipid profiles, and help normalize blood pressure.40-42

Animal studies show that sesame lignans enhance the burning of fat. One study demonstrated that sesame increases the activity of several liver enzymes that break down fatty acids.43 Optimizing the liver’s fat-burning capacity may promote fat loss and may account for sesame’s lipid-lowering effect.

Sesame lignans also boost the weight-loss effects of CLA. Japanese scientists studied whether dietary manipulations could enhance CLA’s effects in reducing body fat. They found that sesamin helped stimulate the loss of adipose tissue. Researchers think that sesame lignans increase CLA’s effects by stimulating a pathway of fatty acid breakdown called beta-oxidation.44

The combination of CLA with sesamin is also effective in lowering serum triglycerides, according to another research team. Triglycerides may increase the risk of heart disease and stroke. Enhanced fatty-acid oxidation in the liver may be responsible for the triglyceride-lowering effect of CLA and sesamin.45

CLA's anti cancer effect

Over the past 18 years, numerous medical and nutritional scientists have published research that validates and expands on the Wisconsin team’s groundbreaking work. Thanks to their efforts, we now know that CLA isomers, when added to the diets of laboratory animals, protect the animals against experimentally induced breast cancer, colon tumors, and skin cancer. These effects occurred regardless of whether the CLA was given during or after tumor initiation.3,5,24,25

Mounting evidence suggests that CLA’s anti-tumor activity comes primarily from the c9t11 isomer. The evidence for CLA’s ability to decrease the incidence, progression, tumor burden, and number of metastases in models of breast, prostate, skin, and colon cancer had grown so convincing by the mid-1990s that the National Academy of Sciences published a statement in 1996 declaring, “[CLA] is the only fatty acid shown unequivocally to inhibit carcinogenesis in experimental animals.”26

One of the animal models used in CLA research routinely serves as a model of a type of human breast cancer called ductal carcinoma in situ.5,24 The finding that CLA inhibits the formation of new tumors in rodents suggests that it may also reduce breast cancer metastasis in women. Confirming this expectation is an important new goal for oncology researchers, as the growth of secondary tumors (metastasis) is the leading cause of death among cancer patients.5

CLA also inhibits the growth of transplanted breast and prostate cancer cell lines in laboratory rodents.27,28 This effect was so dramatic that one research team was inspired to write, “Mice fed the CLA-supplemented diet displayed not only smaller local tumors than the regular diet-fed group, but also a drastic reduction in lung metastases.”28 Considerable evidence indicates that CLA inhibits the initiation stages of cancer, and research also shows that CLA inhibits the post-initiation and promotion phases of cancer growth.5 At least one study found that CLA inhibits the formation of secondary tumors in mice, even after cancerous mammary cells are seeded into the mice.29

In fact, recent evidence suggests that CLA fights cancer at every step in its progression, beginning with its initial development. By blocking cell cycle proteins that regulate the creation of new cells, CLA has been shown to stop runaway cell proliferation before aberrant (but benign) tissue transforms into far-from-benign cancer.5,30,31

In one experiment, researchers fed mice fed CLA for two weeks before inoculating them with aggressive human breast adenocarcinoma cells. Although the mice used in the study were bred for their inability to resist immune challenges, the CLA-treated mice developed 73% fewer local tumors by nine weeks after inoculation than did the control mice, which had not been pre-treated with dietary CLA. Furthermore, CLA dramatically inhibited the spread of breast cancer cells to lungs, peripheral blood, and bone marrow. The researchers concluded, “These results indicate the ability of dietary CLA to block both the local growth and systemic spread of human breast cancer via mechanisms independent of the host immune system.”27

Other research has shown that in a rodent model of human breast cancer, dietary exposure to CLA while mammary glands are maturing confers protection against the cancer. Even when CLA was withdrawn from the diet after mammary glands had matured, rats previously nourished with CLA continued to enjoy significant protection against mammary tumors. The mechanism by which CLA protects in this instance appears to be distinct from its anti-initiation activity.24,32

More recently, scientists in western New York gathered data from more than 1,100 women with confirmed breast cancer, as well as from about 2,000 cancer-free control patients. By assessing the subjects’ self-reported dietary intake of CLA, the researchers were able to look for statistical relationships between CLA intake and health status. Although no clear correlation appeared to exist between dietary intake of CLA and overall breast cancer risk, “a marginally significant reduction in risk of having an [estrogen receptor-negative] tumor” was reported among women with the highest intake of CLA. Regarding CLA, the researchers noted, “There may be associations with tumor biology at least among premenopausal women.”33

Recent reports indicate that CLA may also protect against breast and other types of cancer by inhibiting angiogenesis, the process by which tumors create a new blood supply network to obtain nutrients needed for growth. Halting or reversing angiogenesis is an anti-cancer strategy that has received intense attention by researchers from a variety of disciplines in recent years.34,35


CLA also promotes apoptosis (programmed cell death, or cellular suicide) of cancer cells.36-38 Cancer cells are adept at evading apoptosis, which accounts for much of their insidious nature. Apoptosis allows the body to remove damaged or potentially harmful cells in a way that minimizes waste and ameliorates damage to surrounding tissues. CLA evidently affects the functioning of immune system components involved in apoptosis.38

Recently, a research team in Argentina published a report on diet and the incidence of colon cancer among the local population. Argentineans consume a great deal of beef that is rich in saturated fat and cholesterol, while eating relatively little fish or dietary fiber. Fiber is generally recognized as protective against colon cancer, while heart-healthy omega-3 fatty acids, which are present primarily in seafood, may also help prevent cancer. Despite the Argentineans’ seemingly unhealthy eating habits, they do not succumb to colon cancer as commonly as might be expected.39

An analysis of epidemiological data revealed that high consumption of lean meats (less that 15% fat content) is actually associated with a significant reduction in the incidence of colon cancer among the populace. By contrast, high consumption of fatty meats, such as cold cuts and sausages, was associated with an increased risk of developing colon cancer. Because lean beef is a primary source of CLA, the researchers reasoned that CLA’s protective effects outweigh any presumed negative effects associated with the saturated fats and cholesterol in lean beef.39

Intentionally consuming more beef in order to obtain CLA is not a practical method of protecting one’s health. Most beef comes from cattle that are commercially fed and therefore have relatively little CLA. Numerous scientific studies indicate that those who over-consume beef have higher risks of common diseases.46-49 Those seeking to obtain CLA should consider supplements that provide standardized CLA concentrations.

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